Optimization of Protein Quality Assay in Normal, opaque-2, and Quality Protein Maize
نویسندگان
چکیده
The development of quality protein maize (QPM) was considered a significant leap toward improvement in the nutritional status rural masses developing countries. QPM is attributed to higher concentration essential amino acids, particularly lysine and tryptophan, its kernel endosperm. However, similarity grains normal necessitates standard protocol assess maize. present study aimed at improving assessment QPM. For this purpose, endosperm defatting estimation procedures were restandardized optimized with respect duration amenability for high-throughput analysis. Unlike maize, opaque-2 mutants completely defatted within 48 h period. It observed that tryptophan content, calculated each interval, increased samples longer duration. No differences content analyzed 72 h. Moreover, estimated by using Bradford method certain modifications strongly correlated micro-Kjeldahl ( r = 0.9). Relative method, found be precise, rapid, hazard-free. findings enable testing reduced time can used resource-poor settings determination assay Overall, effectively helped reducing 24 3 as compared already established International Maize Wheat Improvement Center protocol. This expected aggregation high-protein-quality facilitate commercialization.
منابع مشابه
Kernel composition, starch structure, and enzyme digestibility of opaque-2 maize and quality protein maize.
Objectives of this study were to understand how opaque-2 (o2) mutation and quality protein maize (QPM) affect maize kernel composition and starch structure, property, and enzyme digestibility. Kernels of o2 maize contained less protein (9.6-12.5%) than those of the wild-type (WT) counterparts (12.7-13.3%). Kernels of a severe o2 mutant B46o2 also contained less starch (66.9%) than those of B46w...
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Maize (Zea mays L.) plays a very important role in human and animal nutrition in a number of developed and developing countries, worldwide. Breeding for improved protein quality in maize began in the mid-1960s with the discovery of mutants, such as opaque-2, that produce enhanced levels of lysine and tryptophan, the two amino acids deficient in maize endosperm proteins. However, adverse pleiotr...
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Quality Protein Maize (QPM) has about twice the amount of lysine and tryptophan of normal maize and hence represents an important tool of correcting its deficiency in protein quality. However, the effects of low nitrogen and drought on genetic parameters such as gene action and combining abilities of protein quantity and quality of QPM are not known. To study how these genetic parameters are af...
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ژورنال
عنوان ژورنال: Frontiers in sustainable food systems
سال: 2022
ISSN: ['2571-581X']
DOI: https://doi.org/10.3389/fsufs.2022.743019